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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
doi: 10.3389/fphar.2021.628621
Figure Lengend Snippet: Isorhamnetin induces GBC cell apoptosis. (A) The stained NOZ and GBC-SD cells exposed to isorhamnetin (0, 40, 60, and 80 μM). (B) Apoptotic cell proportions are expressed as the mean ± SD. (C) The apoptotic nuclear morphology alterations were assessed using Hoechst 33342 staining via a fluorescence microscope. (D) The expression and (E) statistic results of cleaved PARP, PARP, BAX, cleaved caspases 9, caspases 9, BCL-2, cleaved caspases 3, and caspases 3 in NOZ and GBC-SD after isorhamnetin treatment. * p < 0.05, ** p < 0.01 relative to the control group.
Article Snippet: The membranes were then blocked using 5% skimmed milk at room temperature for 1 h. Thereafter, the membranes were incubated with the primary antibodies cleaved PARP (Cell Signaling Technology Cat# 5625, RRID:AB_10699459), BCL2 (Abcam Cat# ab32124, RRID:AB_725644),
Techniques: Staining, Fluorescence, Microscopy, Expressing
Journal: Frontiers in Pharmacology
Article Title: Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
doi: 10.3389/fphar.2021.628621
Figure Lengend Snippet: Isorhamnetin inhibits GBC cell proliferation and metastasis via the PI3K/AKT pathway. After pretreatment with SC79 for 1 h, the expression levels of (A) MMP-2, Slug, MMP-9, E-cadherin, N-cadherin, TWIST1, (B) cleaved PARP, PARP, BAX, cleaved caspases 9, caspases 9, BCL-2, cleaved caspases 3, caspases 3, (C) cyclin B1, CDK1, p27, p53, p-PI3K, p-AKT1, PIK3CA, and AKT were detected and shown with statistic results. * p < 0.05, ** p < 0.01, *** p < 0.001 relative to the control group.
Article Snippet: The membranes were then blocked using 5% skimmed milk at room temperature for 1 h. Thereafter, the membranes were incubated with the primary antibodies cleaved PARP (Cell Signaling Technology Cat# 5625, RRID:AB_10699459), BCL2 (Abcam Cat# ab32124, RRID:AB_725644),
Techniques: Expressing
Journal: Frontiers in Pharmacology
Article Title: Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation
doi: 10.3389/fphar.2021.628621
Figure Lengend Snippet: Isorhamnetin inhibits tumor growth in vivo . (A,B) We subcutaneously administered NOZ cells into the right flank of nude mice. We then intraperitoneally administered 0.2 ml of vehicle (1% dimethyl sulfoxide and 99% corn oil) or isorhamnetin (1, 5 mg/kg) to the mice every 2 days for 14 days. Necropsy images of mice and tumors were then presented. (C,D) Tumor weight and size measured after tumor harvesting. *** p < 0.001 relative to the control group. (E) The body weight of mice was monitored during the experiment period. (F,G) Expression levels of p-PI3KP85α/γ/β, PIK3CA, cleaved PARP, PARP, cleaved caspases 9, caspases 9, p27, cleaved caspases 3, caspases 3, p53, BAX, BCL-2, N-cadherin, Slug, CDK1, p-AKT1, and AKT were detected. (H) IHC staining of Ki-67 and p-AKT1 expressions in subcutaneous xenograft tumors (magnification = 400 x) ** p < 0.01, *** p < 0.001, **** p < 0.0001 relative to the control group.
Article Snippet: The membranes were then blocked using 5% skimmed milk at room temperature for 1 h. Thereafter, the membranes were incubated with the primary antibodies cleaved PARP (Cell Signaling Technology Cat# 5625, RRID:AB_10699459), BCL2 (Abcam Cat# ab32124, RRID:AB_725644),
Techniques: In Vivo, Expressing, Immunohistochemistry